Cancer Management and Research (Dec 2019)
CircCDYL Serves as a New Biomarker in Mantle Cell Lymphoma and Promotes Cell Proliferation
Abstract
Mei Mei,1,2 Yingjun Wang,1 Qilong Wang,1 Yueyao Liu,3 Wenting Song,1 Mingzhi Zhang1 1Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of China; 2The Academy of Medical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China; 3Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People’s Republic of ChinaCorrespondence: Mingzhi ZhangDepartment of Oncology, The First Affiliated Hospital of Zhengzhou University, No.1 East Jianshe Road, Zhengzhou, People’s Republic of ChinaTel +86 138 3856 5629Fax +86 371 6629 5561Email [email protected]: Mantle cell lymphoma (MCL) is a rare subtype of B-cell lymphoma. Circular (circ) RNA is a member of the non-coding RNA family. However, clinical references to circRNAs in MCL are not clear.Methods: In this study, we detected the expression level of circCDYL in the plasma of MCL patients compared to healthy donors by the quantitative reverse transcription polymerase chain reaction. The diagnostic value of circCDYL was determined using a receiver operating characteristic (ROC) curve. We constructed a circCDYL short hairpin RNA plasmid and infected the MCL cell line, Z138, to detect its effect on cell proliferation.Results: CircCDYL was high expressed in the plasma of MCL patients. The ROC curve showed that circCDYL had diagnostic value (area under the curve (AUC) = 0.856). Functionally, circCDYL knockdown inhibited MCL cell proliferation. We conducted bioinformatics analyses and identified a circCDYL-micro (mi)RNA–mRNA/long non-coding (lnc)RNA network, highlighted by five miRNAs (hsa-miR-129-5p, hsa-miR-3163, hsa-miR-4662a-5p, hsa-miR-101-3p, and hsa-miR-186-5p), three lncRNAs (MALAT1, NEAT1, and XIST), and five mRNAs (NOTCH1, FMR1, ABCB1, TWIST1, and VEGFA).Conclusion: These findings indicate that circ-CDYL might serve as a potential diagnostic biomarker in clinical practice.Keywords: circular RNA, biomarker, AUC, diagnosis, non-coding RNA
