HtrA1 expression associated with the occurrence and development of esophageal cancer

Abstract

Abstract Background The purposes of this study were to measure both the mRNA and protein expression levels of high-temperature requirement serine peptidase 1 (HtrA1) in human esophageal cancer tissues and their adjacent, comparatively normal esophageal tissues. Methods The expression levels of HtrA1 mRNA and protein in both tissue types were measured by semi-quantitative RT-PCR (reverse transcription-polymerase chain reaction) and Western blotting. The clinical and pathological correlation between HtrA1 expression levels and the occurrence and development of esophageal cancer was analyzed. Results The expression levels of HtrA1 mRNA and protein in esophageal carcinoma were significantly lower than the levels expressed in their adjacent normal esophageal tissue (p p p p Conclusions HtrA1 expression is associated with the occurrence and development of esophageal cancer.

Keywords

• Esophageal cancer
• HtrA1 expression
• Semi-quantitative RT-PCR
• SiRNA