Guangxi Zhiwu (Jul 2023)
Tissue-specificity and pathogen-resistant function in vitro of endophytic fungal microbiome harbored in Sophora tonkinensis from wild type
Abstract
The beneficial microbiome assist the plant in disease defense. The root, stem and seed of Sophora tonkinensis from wild type were germinated and grew healthily, but they in cultivated type were susceptible to insect and pathogen attack. In order to explore the possibility of disease control using beneficial endophytic fungal microbiome harbored in S. tonkinensis from healthy wild type, endophytic fungi were isolated from the root, stem and seed of S. tonkinensis from healthy wild type, and then were further identified based on morphological characters and ITS sequence characters. Phylogenetic tree, α-diversity index and β-diversity index were used to analyze the phylogenetic evolution, diversity and similarity among different endophytic fungal microbiomes. The pathogen-resistant function in vitro of endophytic fungal microbiome was determined by the agar plug method and flat-stand method. The results were as follows: (1) A total of 131 strains with 23 taxa, 108 strains with 23 taxa, and 64 strains with 11 taxa, were respectively isolated and identified from the root, stem and seed of S. tonkinensis; (2) More endemic genera and all endemic species, indicated that endophytic fungal microbiomes harbored in the root, stem and seed had tissue specificity in genus and species evolutionary tree; (3) The extremely low β-diversity between root-stem, root-seed, and stem-seed indicated that species similarity was very low among different endophytic fungal microbiomes; (4) The high α-diversity showed that endophytic fungal microbiomes harbored in the root, stem and seed were abundant biodiversity; (5) More than one third of the taxa antagonized pathogens in vitro. Endophytic fungal microbiome harbored in the root/stem showed strong broad-spectrum pathogenic bacteria/fungi-resistant function in vitro, and endophytic fungal microbiome harbored in the seed showed strong broad-spectrum pathogenic fungi-resistant function in vitro. These results indicated beneficial endophytic fungal microbiome with tissue-specificity, biodiversity structure, and strong broad-spectrum pathogen-resistant function in vitro, harbored in healthy root, stem and seed of S. tonkinensis from wild type, and might play an important role in pathogen resistance in host tissues. Such results will provide material and experimental basis for disease control in different tissues of S. tonkinensis using beneficial endophytic fungal microbiome.
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