Biological soil crusts alleviate the stress of arsenic on rice germination and the underlying immobilization mechanisms

Abstract

The high concentration of arsenic (As) in paddy soil has seriously threatened the growth of rice and human food safety. Biological soil crusts (BSC), which are ubiquitous in paddy fields, have been shown a high ability to capture trace metal elements. In the present study, we investigated the effectiveness and mechanism of BSC for immobilizing As, and tested their potential to alleviate the stress of As on rice germination. It is found that BSC can remove 77.8% of arsenic in solution with 3.5 mg L−1 initial As concentration. The As content in BSC reached 514.5 mg kg−1 after 216 h exposure, and the entrapped As was mainly distributed in BSC as a non-EDTA-exchangeable fraction, which might be intracellularly accumulated. Proteobacteria and Bacteroidetes were the dominant phyla in BSC after being exposed to As, playing a significant role in tolerating As and As biogeochemical cycling. The presence of BSC notably promoted the germination rate (18.3%) and dry biomass (103.4%) of rice seeds under 3.5 mg L−1 As stress while reducing the As content in plant roots (8.2–34.3%) and shoots (8.7–47.6%). These findings demonstrate that BSC have a great entrapping effect on As and highlight the importance of BSC in alleviating the stress on rice germination by As, providing a potential nature-based and low-cost strategy to decontaminate paddy fields polluted with As.

Keywords

• Arsenic
• Bacteria diversity
• Biological soil crusts
• Bioremediation
• Distribution