Generation of a human iPSC line (CHCMUi002-A) from peripheral blood mononuclear cells from a chronic granulomatous disease patient

Anle Zeng; Ke Ni; Shuyu Fang; Lang Yu; Yelei Gao; Lina Zhou; Xiaodong Zhao

Stem Cell Research (Feb 2024)

Generation of a human iPSC line (CHCMUi002-A) from peripheral blood mononuclear cells from a chronic granulomatous disease patient

Anle Zeng,
Ke Ni,
Shuyu Fang,
Lang Yu,
Yelei Gao,
Lina Zhou,
Xiaodong Zhao

Affiliations

Anle Zeng: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China
Ke Ni: Aldit Therapeutics, Hangzhou, Zhejiang, China
Shuyu Fang: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China
Lang Yu: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China
Yelei Gao: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China
Lina Zhou: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China
Xiaodong Zhao: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China; Division of Rheumatology and Immunology, Children’s Hospital of Chongqing Medical University, Chongqing, China; Corresponding author at: Children’s Hospital of Chongqing Medical University, Chongqing Key Laboratory of Child Infection and Immunity, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China.

Journal volume & issue: Vol. 74
p. 103269

Abstract

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Chronic granulomatous disease (CGD) is a rare X-linked recessive primary immunodeficiency disease (PID). Herein, a human induced pluripotent stem cell (iPSC) line was generated from the peripheral blood mononuclear cells (PBMCs) of a CGD patient with a mutation (c.785_786delTT) in the CYBB gene. These iPSCs showed the expression of pluripotency markers, the ability to differentiate into three germ layers. They offer a promising technique for studying the pathogenesis and conducting drug screening for CGD patients.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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