Nature Communications (Aug 2023)
Comparative genomic analyses reveal the genetic basis of the yellow-seed trait in Brassica napus
- Cunmin Qu,
- Meichen Zhu,
- Ran Hu,
- Yongchao Niu,
- Si Chen,
- Huiyan Zhao,
- Chengxiang Li,
- Zhen Wang,
- Nengwen Yin,
- Fujun Sun,
- Zhiyou Chen,
- Shulin Shen,
- Guoxia Shang,
- Yan Zhou,
- Xingying Yan,
- Lijuan Wei,
- Liezhao Liu,
- Bin Yi,
- Jinmin Lian,
- Jiang Li,
- Zhanglin Tang,
- Ying Liang,
- Xinfu Xu,
- Rui Wang,
- Jiaming Yin,
- Huafang Wan,
- Hai Du,
- Wei Qian,
- Yourong Chai,
- Qingyuan Zhou,
- Yajun He,
- Silin Zhong,
- Xiao Qiu,
- Hao Yu,
- Hon-Ming Lam,
- Kun Lu,
- Fuyou Fu,
- Jiana Li
Affiliations
- Cunmin Qu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Meichen Zhu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Ran Hu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Yongchao Niu
- The State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong
- Si Chen
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Huiyan Zhao
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Chengxiang Li
- Department of Biological Sciences, Faculty of Science, National University of Singapore
- Zhen Wang
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Nengwen Yin
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Fujun Sun
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Zhiyou Chen
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Shulin Shen
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Guoxia Shang
- National Key Laboratory Breeding Base for Innovation and Utilization of Plateau Crop Germplasm, Academy of Agricultural and Forestry Sciences, Qinghai University
- Yan Zhou
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Xingying Yan
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Lijuan Wei
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Liezhao Liu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Bin Yi
- National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University
- Jinmin Lian
- Biozeron Shenzhen, Inc
- Jiang Li
- Biozeron Shenzhen, Inc
- Zhanglin Tang
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Ying Liang
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Xinfu Xu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Rui Wang
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Jiaming Yin
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Huafang Wan
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Hai Du
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Wei Qian
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Yourong Chai
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Qingyuan Zhou
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Yajun He
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Silin Zhong
- The State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong
- Xiao Qiu
- Department of Food and Bioproduct Sciences, University of Saskatchewan
- Hao Yu
- Department of Biological Sciences, Faculty of Science, National University of Singapore
- Hon-Ming Lam
- The State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong
- Kun Lu
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- Fuyou Fu
- Agriculture and Agri-Food Canada, Saskatoon Research Centre
- Jiana Li
- Engineering Research Center of South Upland Agriculture, Ministry of Education, College of Agronomy and Biotechnology, Southwest University
- DOI
- https://doi.org/10.1038/s41467-023-40838-1
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 16
Abstract
Abstract Yellow-seed trait is a desirable breeding characteristic of rapeseed (Brassica napus) that could greatly improve seed oil yield and quality. However, the underlying mechanisms controlling this phenotype in B. napus plants are difficult to discern because of their complexity. Here, we assemble high-quality genomes of yellow-seeded (GH06) and black-seeded (ZY821). Combining in-depth fine mapping of a quantitative trait locus (QTL) for seed color with other omics data reveal BnA09MYB47a, encoding an R2R3-MYB-type transcription factor, as the causal gene of a major QTL controlling the yellow-seed trait. Functional studies show that sequence variation of BnA09MYB47a underlies the functional divergence between the yellow- and black-seeded B. napus. The black-seed allele BnA09MYB47aZY821, but not the yellow-seed allele BnA09MYB47aGH06, promotes flavonoid biosynthesis by directly activating the expression of BnTT18. Our discovery suggests a possible approach to breeding B. napus for improved commercial value and facilitates flavonoid biosynthesis studies in Brassica crops.
