The Sequencing of hpmB Gene in Proteus mirabilis Among UTIs Patients

Abstract

The present study was carried out during February to May 2018 in Baghdad hospitals. A sample of urine has been collected from fifty patients with an infection in their urinary tract (UTIs) of both sexes and different ages. Bacteriological investigation of urine samples from UTIs patients is made to isolate and diagnose Proteus mirabilis bacterium. In addition, the study detects the phenotypic and genetic characteristic of Proteus mirabilis α-hemolysin activity. Moreover, for the study to prove its hypothesis, a molecular detection has been carried out utilizing specific primer to hpmB gene which encodes α-Hemolysin as a factor of virulence of Proteus mirabilis through the use of PCR. The results show that 7(%100) of isolates are positive for hpmB at 422 bp. Two isolates of P. mirabilis are sequenced as hpmB genes. The ratio of identity of the hpmB genes with the CP017085.1 and CP020052.1 stains at NCBI global databases copmrised 100%, 99% respectively.

Keywords

• p. mirabilis
• virulence factors gene hpmb
• sequencing.