Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases

M. A. Jackson; E. K. Gilding; T. Shafee; K. S. Harris; Q. Kaas; S. Poon; K. Yap; H. Jia; R. Guarino; L. Y. Chan; T. Durek; M. A. Anderson; D. J. Craik

doi:10.1038/s41467-018-04669-9

Nature Communications (Jun 2018)

Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases

M. A. Jackson,
E. K. Gilding,
T. Shafee,
K. S. Harris,
Q. Kaas,
S. Poon,
K. Yap,
H. Jia,
R. Guarino,
L. Y. Chan,
T. Durek,
M. A. Anderson,
D. J. Craik

Affiliations

M. A. Jackson: Institute for Molecular Bioscience, The University of Queensland
E. K. Gilding: Institute for Molecular Bioscience, The University of Queensland
T. Shafee: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
K. S. Harris: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
Q. Kaas: Institute for Molecular Bioscience, The University of Queensland
S. Poon: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
K. Yap: Institute for Molecular Bioscience, The University of Queensland
H. Jia: Institute for Molecular Bioscience, The University of Queensland
R. Guarino: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
L. Y. Chan: Institute for Molecular Bioscience, The University of Queensland
T. Durek: Institute for Molecular Bioscience, The University of Queensland
M. A. Anderson: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University
D. J. Craik: Institute for Molecular Bioscience, The University of Queensland

DOI: https://doi.org/10.1038/s41467-018-04669-9
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

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Asparaginyl endopeptidases (AEPs) are plant proteases that can also function as ligases, catalyzing the production of cyclic plant peptides. Here, the authors identify structural features that govern AEP ligase activity, providing insights to aid the discovery and engineering of ligase-type AEPs.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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