Infection and Drug Resistance (Mar 2024)
Isolation, and Identification of Escherichia coli O157:H7 Recovered from Chicken Meat at Addis Ababa Slaughterhouses
Abstract
Hailehizeb Tegegne,1 Kassahun Filie,1 Tadele Tolosa,2 Motuma Debelo,2 Eyoel Ejigu2 1Department of Veterinary Science, College of Agriculture and Environmental Science, Debre Tabor University, Debre Tabor, Amhara, Ethiopia; 2Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine Jimma University, Jimma, Oromia, EthiopiaCorrespondence: Hailehizeb Tegegne, Email [email protected]: It is a growing concern that pathogenic bacteria can be found in poultry meat, and an additional problem is the ongoing rise in food-derived bacteria’s resistance to antibiotics.Methods: A cross-sectional study was conducted from February 2021 to March 2023. This study aimed to isolate, identify and analyze E. coli in poultry meat collected from slaughterhouses in Addis Ababa. A total of 499 samples were collected from fecal content, carcass, hand and knife swab samples by using purposive and systematic random sampling methods.Results: An overall 5.2% Escherichia coli (E. coli O157:H7) prevalence was found in this study. On average, antimicrobial susceptibility tests revealed that the sensitivities of (Escherichia coli) E. coli O157:H7 isolates from carcass swabs, fecal content, manuals, knife swabs and water samples were 62.3%, 52.5%, 53%, 60% and 60%, respectively.Discussion: However, few (Escherichia coli) E. coli O157:H7 isolates have developed multidrug resistance to some antibiotics, for an overall rate of 13.8%. Knowledge and attitude assessment of the slaughterhouse workers showed that 44.2% had no wash their hands at all and that 125.6% had washed their hands before slaughter commencement.Conclusion: The detection of (Escherichia coli) E. coli O157:H7 isolates from all sample types had a slight prevalence of serious public health concerns. It is important to monitor antibiotic use for both human and animal health in order to make sure that it is safe and effective.Keywords: Addis Ababa, antibiogram, chicken, Escherichia coli, slaughterhouse
