Frontiers in Neuroscience (Jan 2021)
SPARC Knockdown Reduces Glutamate-Induced HT22 Hippocampal Nerve Cell Damage by Regulating Autophagy
- Shuang Chen,
- Shuang Chen,
- Qin Zou,
- Qiang Guo,
- Yongmin Chen,
- Yongmin Chen,
- Xi Kuang,
- Xi Kuang,
- Yukang Zhang,
- Yukang Zhang,
- Yan Liu,
- Yan Liu,
- Wengang Wu,
- Wengang Wu,
- Ge Li,
- Ge Li,
- Linzhi Tu,
- Linzhi Tu,
- Jingyi Tong,
- Jingyi Tong,
- Songrong Li,
- Songrong Li,
- Lin Ma,
- Lin Ma,
- Qifu Li,
- Qifu Li
Affiliations
- Shuang Chen
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Shuang Chen
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Qin Zou
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Qiang Guo
- Epilepsy Center, Guangdong Sanjiu Brain Hospital, Guangzhou, China
- Yongmin Chen
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Yongmin Chen
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Xi Kuang
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Xi Kuang
- Hainan Health Vocational College, Haikou, China
- Yukang Zhang
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Yukang Zhang
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Yan Liu
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Yan Liu
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Wengang Wu
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Wengang Wu
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Ge Li
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Ge Li
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Linzhi Tu
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Linzhi Tu
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Jingyi Tong
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Jingyi Tong
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Songrong Li
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Songrong Li
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Lin Ma
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Lin Ma
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- Qifu Li
- Department of Neurology, The First Affiliated Hospital of Hainan Medical University, Haikou, China
- Qifu Li
- Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou, China
- DOI
- https://doi.org/10.3389/fnins.2020.581441
- Journal volume & issue
-
Vol. 14
Abstract
Secreted protein acidic and rich in cysteine (SPARC) is a matricellular protein involved in the extracellular matrix and interactions between cells during neural development of the central nervous system (CNS). Oxidative glutamate toxicity is involved in CNS diseases, including epilepsy, Alzheimer’s disease, and ischemic stroke. However, the molecular mechanism of nerve injury is not fully understood in CNS diseases. Herein, the glutamate-induced nerve damage model was used to explore the molecular mechanisms affecting nerve damage. The levels of SPARC and autophagy were increased in glutamate-induced HT22 hippocampal nerve injury. In summary, the current study confirmed that SPARC regulates autophagy in HT22 hippocampal nerve cells, and its knockdown reduces the glutamate-induced HT22 hippocampal nerve injury by inhibiting autophagy. These findings suggested that SPARC plays a crucial role in nerve injury of CNS diseases.
Keywords
