Isolation and characterization of plant growth-promoting bacteria from Syrian bean caper (Zygophyllum fabago) rhizosphere

Abstract

Some rhizobacteria have positive effects on plants growth. Syrian bean-caper (Zygophyllum fabago) is a weed plant with medicinal value. This study was conducted to isolate and identify bacteria from Syrian bean-caper rhizosphere. Characteristics associated with plant growth stimulation, such as phosphate and zinc dissolution, production of Indole acetic acid and antifungal activity, were investigated. The isolates were separately inoculated to the plant and after plant root establishment was ensured, their effectiveness in increasing plant growth in greenhouse conditions was measured. Biochemical and molecular identification results showed that five isolates belonged to the genera Bacillus, Pseudomonas, Pantoea, and Brevibacterium. All five isolates showed some degree of plant growth promotion capabilities. Among the isolates, only the genus Bacillus increased the dry weights of plants significantly. The amount of phosphate solubilization for this isolate was 440 μg ml-1 and its acid production in the culture medium was higher than that in other isolates. The isolate had zinc solubilisation capability and produced 3.89 mg ml-1 indole acetic acid. However, this isolate did not show antifungal activity against two fungal pathogens of Aspergillus niger and Botrytis cinerea.

Keywords

• antifungal activity
• bacteria
• indole-acetic acid
• phosphate solubilization
• root