Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe

Troy F. Langford; Beijing K. Huang; Joseph B. Lim; Sun Jin Moon; Hadley D. Sikes

doi:10.1038/s41467-018-05557-y

Nature Communications (Aug 2018)

Monitoring the action of redox-directed cancer therapeutics using a human peroxiredoxin-2-based probe

Troy F. Langford,
Beijing K. Huang,
Joseph B. Lim,
Sun Jin Moon,
Hadley D. Sikes

Affiliations

Troy F. Langford: Department of Chemical Engineering, Massachusetts Institute of Technology
Beijing K. Huang: Department of Biological Engineering, Massachusetts Institute of Technology
Joseph B. Lim: Department of Chemical Engineering, Massachusetts Institute of Technology
Sun Jin Moon: Department of Chemical Engineering, Massachusetts Institute of Technology
Hadley D. Sikes: Department of Chemical Engineering, Massachusetts Institute of Technology

DOI: https://doi.org/10.1038/s41467-018-05557-y
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 12

Abstract

Read online

Current sensors for intracellular H2O2 are not sensitive enough to detect all biologically relevant H2O2 fluctuations. Here the authors develop a peroxiredoxin-2-based FRET probe that is sensitive enough to measure changes in H2O2 concentration in response to the cancer therapeutic piperlongumine.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal