Bioconversion of black rice and blueberry regulate immunity system through regulation of MAPKs, NF-kB in RAW264.7 macrophage cells

Abstract

Bioconversion can enhance the functionality of efficacy. We made bioconversion material with black rice (Oryza sativa L.) and blueberry (Vaccinium crymbosum L.), using Lactobacillus plantarum (call BB). In this study, BB investigated anti-inflammatory and immune-boosting effects through immune system control. Therefore, we investigated the efficacy of BB in the presence and absence of LPS (Lipopolysaccharide). In the presence of LPS, the bioconversion material inhibited nitric oxide (NO) production from LPS in macrophages, inhibited inducible nitric oxide synthase (iNOS), Cyclooxygenase 2 (COX-2), phosphorylation of mitogen-activated protein kinases (such as JNK, ERK and p-38) and reduced the secretion of cytokines Tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6). In the absence of LPS, BB exhibited an effect that was opposite of LPS stimulation. These data show that the effect was lower than that of LPS. In conclusion, BB suggested that anti-inflammatory and immune-boosting effects through the regulation of the immune system are multi-efficacy materials.

Keywords

• bioconversion
• anti-inflammatory
• immune-boosting
• black rice
• blueberry
• lactobacillus plantarum