Association of wnt9b rs1530364 and wnt5a rs566926 gene polymorphisms with nonsyndromic cleft lip and palate in South Indian population using deoxyribonucleic acid sequencing

Abstract

Context: Nonsyndromic cleft lip with or without cleft palate (CL/CP) is a common congenital facial malformation without any other structural or developmental abnormalities. Aims and Objectives: To test the association of Wnt9B rs1530364 and Wnt5A rs566926 gene variants with the nonsyndromic CL/CP patients in South Indian population. Methods: Deoxyribonucleic acid (DNA) samples of 25 subjects with nonsyndromic cleft lip and palate (NSCLP) and 25 unrelated controls collected from the department were used for the study. Group A: DNA samples of 25 subjects NSCLP (P1–P25). Group B: DNA samples of 25 unrelated controls (C1–C25). The extracted DNA samples were subjected to polymerase chain reaction, and later, these amplified products were subjected to DNA sequencing. Results were documented in the form of electropherograms. Results: The results indicated that there is a strong association between the presence of Wnt9B rs1530364 gene with the incidence of NSCLP. This study also suggests that the likelihood of NSCLP is higher in subjects having CC (P = 0.02) genotype for Wnt9B gene variant rs1530364. Conclusion: We can conclude that Wnt9B gene variant rs1530364 can be considered as genetic marker for NSCLP for our population.

Keywords

• nonsyndromic cleft lip and palate
• wnt5a gene variant rs566926
• wnt9b gene variant rs1530364