Mitigation of arsenic accumulation in rice (Oryza sativa L.) seedlings by oxygen nanobubbles in hydroponic cultures

Abstract

Arsenic (As) is a toxic non-essential metal. Its accumulation in rice has not only seriously affected the growth of rice, but also poses a significant threat to human health. Many reports have been published to decrease the arsenic accumulation in the rice plant by various additives such as chemicals, fertilizers, adsorbents, microorganisms and analyzing the mechanism. Nanobubble is a new technology widely used in agriculture because of its long existence time and high mass transfer efficiency. However, a few studies have investigated the effect of nanobubbles on arsenic uptake in rice. This study investigated the effect of oxygen nanobubbles on the growth and uptake of As in rice. The oxygen nanobubbles could rupture the salinity of nutrients and produce the hydroxyl radical. The hydroxyl radical caused the oxidation of arsenic As(III) to As (V) and the oxidation of ferrous ions. At the same time, the oxidized iron adsorbing As (V) created the iron plaque on the rice roots to stop arsenic introduction into the rice plant. The results indicated that the treatment of oxygen nanobubbles increased rice biomass under As stress, while they increased the chlorophyll content and promoted plant photosynthesis. Oxygen nanobubbles reduced the As content in rice roots to 12.5% and shoots to 46.4%. In other words, it significantly decreased As accumulation in rice. Overall, oxygen nanobubbles mitigated the toxic effects of arsenic on rice and had the potential to reduce the accumulation of arsenic in rice.

Keywords

• As
• Hydroxyl radicals
• Iron plaque
• O2 nanobubbles