Biodegradable Cell Microcarriers Based on Chitosan/Polyester Graft-Copolymers
Tatiana S. Demina,
Maria G. Drozdova,
Chantal Sevrin,
Philippe Compère,
Tatiana A. Akopova,
Elena Markvicheva,
Christian Grandfils
Affiliations
Tatiana S. Demina
Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences (ISPM RAS), 70 Profsoyuznaya str., 117393 Moscow, Russia
Maria G. Drozdova
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., 117997 Moscow, Russia
Chantal Sevrin
Interfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), Belgium
Philippe Compère
Interfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), Belgium
Tatiana A. Akopova
Enikolopov Institute of Synthetic Polymeric Materials of Russian Academy of Sciences (ISPM RAS), 70 Profsoyuznaya str., 117393 Moscow, Russia
Elena Markvicheva
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str., 117997 Moscow, Russia
Christian Grandfils
Interfaculty Research Centre on Biomaterials (CEIB), University of Liège, Chemistry Institute, B6C, 11 Allée du 6 août, B-4000 Liege (Sart-Tilman), Belgium
Self-stabilizing biodegradable microcarriers were produced via an oil/water solvent evaporation technique using amphiphilic chitosan-g-polyester copolymers as a core material in oil phase without the addition of any emulsifier in aqueous phase. The total yield of the copolymer-based microparticles reached up to 79 wt. %, which is comparable to a yield achievable using traditional emulsifiers. The kinetics of microparticle self-stabilization, monitored during their process, were correlated to the migration of hydrophilic copolymer’s moieties to the oil/water interface. With a favorable surface/volume ratio and the presence of bioadhesive natural fragments anchored to their surface, the performance of these novel microcarriers has been highlighted by evaluating cell morphology and proliferation within a week of cell cultivation in vitro.
