In Vitro Osteogenic Analysis of a Novel Scaffold for Bone Regeneration

Abstract

Objective: This study aimed to analyse the osteogenic potential of a novel experimental material comprising of nano-hydroxyapatite grafted glass fiber scaffolds using murine osteoblast MC3T3-E1 cell lines through gene expression of biomarkers for osteogenesis. Study Design:In vitro experimental study. Place and Duration of Study: Bone Marrow Transplant Centre and Genetic Resource Centre, Rawalpindi from 10 May 2021 to 17 June 2022. Materials and Methods: Two types of nano-apatite grafted E-glass fiber scaffolds i.e., 10 wt% E-glass/90wt% Hydroxyapatite (E-10), 20% E-glass/80% Hydroxyapatite (E-20) were used, whereby pure nanohydroxyapatite (E-0) was used as control group. A comparative gene expression of Osteopontin (OP) and Collagen (Col) Type 1 was measured through reverse transcription polymerase chain reaction (RT-PCR). Results: Both experimental groups showed expression of the osteogenic proteins. The level of OP expression was higher in E-10 compared to E-20, whereas the level of Col expression was higher in E-20. Only for E-0, Col expression was almost same as OP whereas for the E-10 and E-20, OP expression was greater. Conclusions: Based on the findings, both experimental scaffolds supported the growth of cells and showed an osteogenic nature. It is anticipated that the experimental scaffolds have potential to be used for bone regeneration.

Keywords

• bone regeneration
• e-glass fibres
• gene expression
• hydroxyapatite
• reverse transcription polymerase chain reaction.