Phytomedicine Plus (May 2023)

Morus alba extract suppresses IL-17-induced abnormal proliferation in 3D-reconstructed epidermis

  • Takahiro Oyama,
  • Michiru Usui,
  • Ena Sato,
  • Hideaki Abe,
  • Takanori Kamiya,
  • Takehiko Abe,
  • Sei-ichi Tanuma

Journal volume & issue
Vol. 3, no. 2
p. 100455

Abstract

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Introduction: Psoriasis is a chronic disease that is characterized by the abnormal proliferation of keratinocytes. The pathogenesis of psoriasis is initiated by the infiltration of Th17 cells, which secrete proinflammatory cytokines, including interleukin (IL)-17. IL-17 subsequently affects keratinocytes, causing abnormal proliferation and the release of cytokines and chemokines, which results in a vicious cycle of increased IL-17 production by immune cells. In this study, we aimed to search for a new remedy for treating psoriasis from natural sources. Methods: We screened plant extracts that suppress IL-17-induced chemokine, chemokine (C-C motif) ligand 20 (CCL20) by qRT–PCR from the in-house library. The hit extract was evaluated by a 2D-wound healing assay and a 3D-reconstruction epidermal model. Result: Morus alba extract (MAE) was discovered as a candidate for a suppressor of IL-17-induced expression of CCL20. MAE also suppressed the migration of keratinocytes on wound-healing assay on a 2D culture plate. Additionally, MAE normalized the abnormal proliferation of the 3D-reconstruction model of keratinocytes induced by IL-17. Conclusion: MAE may offer promise as a natural treatment for psoriasis.

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