Determination of Free Histidine in Complex Hair Care Products with Minimum Sample Preparation Using Cation-Exchange Chromatography and Post Column Derivatization

Apostolia Tsiasioti; Constantinos K. Zacharis; Paraskevas D. Tzanavaras

doi:10.3390/molecules28020888

Molecules (Jan 2023)

Determination of Free Histidine in Complex Hair Care Products with Minimum Sample Preparation Using Cation-Exchange Chromatography and Post Column Derivatization

Apostolia Tsiasioti,
Constantinos K. Zacharis,
Paraskevas D. Tzanavaras

Affiliations

Apostolia Tsiasioti: Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Constantinos K. Zacharis: Laboratory of Pharmaceutical Analysis, Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece
Paraskevas D. Tzanavaras: Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece

DOI: https://doi.org/10.3390/molecules28020888
Journal volume & issue: Vol. 28, no. 2
p. 888

Abstract

Read online

In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5–114.8%) and precise (2.4–5.6% RSD) determination of free histidine without matrix interferences at concentration levels down to 1.5 mg kg−1. Real commercially available samples were found to contain the amino acid at levels ranging between 70 and 535 mg kg−1.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

About the journal

Abstract

Keywords