WORKING OUT AND VALIDATION OF THE METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN THE HERB OF LEONTODON AUTUMNALIS L.

Abstract

In the course of the study we have worked out the methodology of differential spectrophotometric determination of flavonoids in raw Leontodon autumnalis L. after the formation of a colored complex with a solution of aluminum chloride. We have established optimal conditions for complexation and extraction of flavonoids from plant raw materials under study. We have established that the most complete extraction of flavonoids is achieved with fineness of 1 mm by extraction with ethyl alcohol of 70% for 45 minutes. The maximum value of the optical density is achieved 30 minutes after addition of 2 ml of a solution of aluminum chloride 2%.

Keywords

• дифференциальная спектрофотометрия
• флавоноиды
• кульбаба осенняя