Study on the Data Collection Method and Application of Standard Sample Database of Yunnan High-quality Rice

Abstract

【Objective】The method of collecting standard sample data of high-quality rice was explored in order to build a standard sample database of high-quality rice varieties in Yunnan Province and provide technical support for authenticity identification, purification and rejuvenation of high-quality rice varieties.【Method】The seeds for production and the stock seeds of Yunhui 290 and Diantun 502 were used as research materials. The characteristics of high-quality rice varieties were comprehensively and completely described by using techniques such as variety agronomic character investigation, SSR molecular marker construction fingerprint, rice quality detection.【Result】Twelve pairs of core markers on 12 chromosomes of rice were used to collect the fingerprints of the seeds for production and the stock seeds. The results showed that there was no difference between the seeds for production and the stock seeds of Yunhui 290 and Diantun 502, which could be used to describe the fingerprints of Yunhui 290 and Diantun 502. 12 pairs of core markers were used to identify the genotype consistency of 48 individual plants randomly selected from the seeds for production and the stock seeds. The seeds for production and stock seeds of Yunhui 290 had the same amplification products in 12 markers, and there were no off-type. The band from the stock seeds of Diantun 502 were consistent, and there were no off-type. There were two markers with abnormal bands and two off-type in the seeds for production of Diantun 502. By using DUS test method, 17 DUS test characters and 4 plant parts pictures were collected, and 11 quality characters were collected according to the national high quality rice standard.【Conclusion】With variety fingerprint, DUS test character, plant part picture, quality character and commodity information as five elements of the database, the standard sample database of high quality rice is built by computer programming software. The establishment of the standard sample database of high-quality rice can systematically describe varieties from the gene level, variety characteristics and appearance.

Keywords

• high-quality rice
• fingerprint
• phenotype trait
• commodity information
• database