Modeling interspecific competition of the microbial community during anaerobic digestion based on cellular automata and ADM1

Abstract

Interspecific competition for substrate and space gives rise to considerable variation in biomass distribution within the microbial community. To study microbial community in depth, we used several research methods as sampling and analytical measurements, and developed a cellular automata (CA) model that would facilitate a description of the microbial growth process based on Anaerobic Digestion Model No. 1 (ADM1) of the International Water Association (IWA). Using the CA model, we aimed to determine whether interspecific competition occurs among acidogens, acetogens and methanogens, and to examine the influence of interspecific competition on the spatial structure of microbial communities. We found that acetogens and methanogens competed for core space, resulting in a multi-layer structure. Butyrate-degrading acetogens increased in number, resulting in inhibition of propionate-degrading acetogens. Hydrogenotrophic methanogens showed stronger competitive advantage than acetotrophic methanogens. The simulation showed that the multi-layer structure of the microbial community was formed by interspecific competition. HIGHLIGHTS A two-dimensional model is developed based on cellular automata and ADM1.; New biomass-spreading rules are introduced to cellular automata.; The integrated model is evaluated by simulation of ABR start-up.; The new model can be used to simulate interspecific competition.;

Keywords

• acetogens
• adm1
• biomass distribution
• cellular automata
• methanogen