Effects of Mango Seed (<i>Mangifera indica</i>) Powder on Growth Performance, Immune Response, Gut Morphology, and Gene Expression of Nile Tilapia (<i>Oreochromis niloticus</i>)
Camilla Maria Fontana,
Md Afsar Ahmed Sumon,
Supreya Wannavijit,
Anisa Rilla Lubis,
Nuttapon Khongdee,
Nguyen Vu Linh,
Yuthana Phimolsiripol,
Seyed Hossein Hoseinifar,
Hien Van Doan
Affiliations
Camilla Maria Fontana
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Md Afsar Ahmed Sumon
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Supreya Wannavijit
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Anisa Rilla Lubis
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Nuttapon Khongdee
Department of Highland Agriculture and Natural Resources, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Nguyen Vu Linh
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Yuthana Phimolsiripol
Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand
Seyed Hossein Hoseinifar
Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan 49189-43464, Iran
Hien Van Doan
Department of Animal and Aquatic Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
This study explored the effects of mango seed (MS) powder supplementation on the growth, immune response, gene expression, and intestinal morphology of Nile tilapia (Oreochromis niloticus) over an 8-week period. A total of 300 Nile tilapia fingerlings (average weight of 15.29 ± 0.05 g) were divided into five treatment groups and fed either a basal diet or one of four experimental diets containing MS powder at concentrations of 10 (MS10), 20 (MS20), 40 (MS40), and 80 (MS80) g kg−1. The results demonstrated that Nile tilapia fed MS-supplemented diets experienced significant improvements (p −1 MS). The MS-treated groups also showed a significant increase (p p IL-1, IL-8, and LBP) and antioxidant genes (GST-α, GPX, and GSR). Polynomial regression analysis identified an optimal MS dosage of 36.43–45 g kg−1 for effectively improving growth, immunity, and immuno-oxidant gene expression in Nile tilapia. These results emphasize mango seed (MS) as a promising natural supplement for improving the diet of Nile tilapia and, potentially, other freshwater fish widely used in aquaculture.
