Simplification of RNA preparation procedure for RT-PCR in detection of pome fruit tree viruses

Abstract

A rapid, easy to handling and sensitive RNA preparation procedure, RNA release protocol was described here for the detection of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) by RT-PCR. Comparing total RNA extraction protocol, RNA release protocol give raised similar rate of ASPV and ASGV detection within the field-grown apple cultivars. Among sampling plant tissues, the bud leaf and leaf (during blossom) were showed efficient tissues for the routine detection, regardless the using RNA preparation procedures.

Keywords

• rt-pcr
• aspv
• asgv
• rna release