Frontiers in Cellular and Infection Microbiology (Jan 2023)

Comparative genome analysis reveals high-level drug resistance markers in a clinical isolate of Mycobacterium fortuitum subsp. fortuitum MF GZ001

  • Md Shah Alam,
  • Md Shah Alam,
  • Md Shah Alam,
  • Md Shah Alam,
  • Ping Guan,
  • Yuting Zhu,
  • Yuting Zhu,
  • Sanshan Zeng,
  • Sanshan Zeng,
  • Sanshan Zeng,
  • Sanshan Zeng,
  • Xiange Fang,
  • Xiange Fang,
  • Xiange Fang,
  • Xiange Fang,
  • Shuai Wang,
  • Shuai Wang,
  • Buhari Yusuf,
  • Buhari Yusuf,
  • Buhari Yusuf,
  • Buhari Yusuf,
  • Jingran Zhang,
  • Jingran Zhang,
  • Jingran Zhang,
  • Jingran Zhang,
  • Xirong Tian,
  • Xirong Tian,
  • Xirong Tian,
  • Xirong Tian,
  • Cuiting Fang,
  • Cuiting Fang,
  • Cuiting Fang,
  • Cuiting Fang,
  • Yamin Gao,
  • Yamin Gao,
  • Yamin Gao,
  • Yamin Gao,
  • Mst Sumaia Khatun,
  • Mst Sumaia Khatun,
  • Mst Sumaia Khatun,
  • Mst Sumaia Khatun,
  • Zhiyong Liu,
  • Zhiyong Liu,
  • Zhiyong Liu,
  • Zhiyong Liu,
  • H. M. Adnan Hameed,
  • H. M. Adnan Hameed,
  • H. M. Adnan Hameed,
  • H. M. Adnan Hameed,
  • Yaoju Tan,
  • Jinxing Hu,
  • Jianxiong Liu,
  • Tianyu Zhang,
  • Tianyu Zhang,
  • Tianyu Zhang,
  • Tianyu Zhang

DOI
https://doi.org/10.3389/fcimb.2022.1056007
Journal volume & issue
Vol. 12

Abstract

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IntroductionInfections caused by non-tuberculosis mycobacteria are significantly worsening across the globe. M. fortuitum complex is a rapidly growing pathogenic species that is of clinical relevance to both humans and animals. This pathogen has the potential to create adverse effects on human healthcare.MethodsThe MF GZ001 clinical strain was collected from the sputum of a 45-year-old male patient with a pulmonary infection. The morphological studies, comparative genomic analysis, and drug resistance profiles along with variants detection were performed in this study. In addition, comparative analysis of virulence genes led us to understand the pathogenicity of this organism.ResultsBacterial growth kinetics and morphology confirmed that MF GZ001 is a rapidly growing species with a rough morphotype. The MF GZ001 contains 6413573 bp genome size with 66.18 % high G+C content. MF GZ001 possesses a larger genome than other related mycobacteria and included 6156 protein-coding genes. Molecular phylogenetic tree, collinearity, and comparative genomic analysis suggested that MF GZ001 is a novel member of the M. fortuitum complex. We carried out the drug resistance profile analysis and found single nucleotide polymorphism (SNP) mutations in key drug resistance genes such as rpoB, katG, AAC(2')-Ib, gyrA, gyrB, embB, pncA, blaF, thyA, embC, embR, and iniA. In addition, the MF GZ001strain contains mutations in iniA, iniC, pncA, and ribD which conferred resistance to isoniazid, ethambutol, pyrazinamide, and para-aminosalicylic acid respectively, which are not frequently observed in rapidly growing mycobacteria. A wide variety of predicted putative potential virulence genes were found in MF GZ001, most of which are shared with well-recognized mycobacterial species with high pathogenic profiles such as M. tuberculosis and M. abscessus.DiscussionOur identified novel features of a pathogenic member of the M. fortuitum complex will provide the foundation for further investigation of mycobacterial pathogenicity and effective treatment.

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