Protocol for electrotaxis of large epithelial cell sheets

Yan Zhang; Rachel M. Lee; Zijie Zhu; Yaohui Sun; Kan Zhu; Zhengping Xu; Francis Lin; Tingrui Pan; Wolfgang Losert; Min Zhao

STAR Protocols (Jun 2023)

Protocol for electrotaxis of large epithelial cell sheets

Yan Zhang,
Rachel M. Lee,
Zijie Zhu,
Yaohui Sun,
Kan Zhu,
Zhengping Xu,
Francis Lin,
Tingrui Pan,
Wolfgang Losert,
Min Zhao

Affiliations

Yan Zhang: Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; School of Public Health, Hangzhou Normal University, Hangzhou 311121, China; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA; Corresponding author
Rachel M. Lee: Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA
Zijie Zhu: Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA
Yaohui Sun: Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA
Kan Zhu: Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA
Zhengping Xu: Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China
Francis Lin: Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
Tingrui Pan: Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA; Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Single-molecule Detection and Instrument Development, Shenzhen, Guangdong 518055, China; Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou 215123, China
Wolfgang Losert: Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA; Department of Physics, University of Maryland, College Park, MD 20742, USA
Min Zhao: Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; Department of Dermatology, University of California, California, Davis, CA 95616, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 2
p. 102288

Abstract

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Summary: Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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