Inhibition of Bacterial Quorum Sensing by Extracts from Aquatic Fungi: First Report from Marine Endophytes
Alberto J. Martín-Rodríguez,
Fernando Reyes,
Jesús Martín,
Juan Pérez-Yépez,
Milagros León-Barrios,
Alan Couttolenc,
César Espinoza,
Ángel Trigos,
Víctor S. Martín,
Manuel Norte,
José J. Fernández
Affiliations
Alberto J. Martín-Rodríguez
Institute for Bioorganic Chemistry "Antonio González", Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain
Fernando Reyes
Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud, Av. del Conocimiento 34, 18016 Armilla, Granada, Spain
Jesús Martín
Fundación MEDINA, Parque Tecnológico de Ciencias de la Salud, Av. del Conocimiento 34, 18016 Armilla, Granada, Spain
Juan Pérez-Yépez
Department of Microbiology, Biochemistry, Cellular Biology and Genetics, Faculty of Health Science, University of La Laguna, Av. Astrofísico Francisco Sánchez s/n, 38071 La Laguna, Tenerife, Spain
Milagros León-Barrios
Department of Microbiology, Biochemistry, Cellular Biology and Genetics, Faculty of Health Science, University of La Laguna, Av. Astrofísico Francisco Sánchez s/n, 38071 La Laguna, Tenerife, Spain
Alan Couttolenc
Ph.D. Program in Biomedical Sciences, Veracruzana University, Luis Castelazo Ayala s/n, Colonia Industrial Ánimas, 91190 Xalapa, Veracruz, Mexico
César Espinoza
Xalapa High Technology Laboratory (LATEX), Veracruzana University, Médicos 5, Unidad del Bosque, 91010 Xalapa, Veracruz, Mexico
Ángel Trigos
Xalapa High Technology Laboratory (LATEX), Veracruzana University, Médicos 5, Unidad del Bosque, 91010 Xalapa, Veracruz, Mexico
Víctor S. Martín
Institute for Bioorganic Chemistry "Antonio González", Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain
Manuel Norte
Institute for Bioorganic Chemistry "Antonio González", Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain
José J. Fernández
Institute for Bioorganic Chemistry "Antonio González", Center for Biomedical Research of the Canary Islands (CIBICAN), University of La Laguna, Av. Astrofísico Francisco Sánchez 2, 38206 La Laguna, Tenerife, Spain
In our search for quorum-sensing (QS) disrupting molecules, 75 fungal isolates were recovered from reef organisms (endophytes), saline lakes and mangrove rhizosphere. Their QS inhibitory activity was evaluated in Chromobacterium violaceum CVO26. Four strains of endophytic fungi stood out for their potent activity at concentrations from 500 to 50 μg mL−1. The molecular characterization, based on the internal transcribed spacer (ITS) region sequences (ITS1, 5.8S and ITS2) between the rRNA of 18S and 28S, identified these strains as belonging to four genera: Sarocladium (LAEE06), Fusarium (LAEE13), Epicoccum (LAEE14), and Khuskia (LAEE21). Interestingly, three came from coral species and two of them came from the same organism, the coral Diploria strigosa. Metabolic profiles obtained by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) suggest that a combination of fungal secondary metabolites and fatty acids could be the responsible for the observed activities. The LC-HRMS analysis also revealed the presence of potentially new secondary metabolites. This is, to the best of our knowledge, the first report of QS inhibition by marine endophytic fungi.
