Zirconium oxide nanoparticles: Protocolicidal effect against hydatid cyst protoscoleces with interleukins evaluation in mice

Abstract

The current study investigated the effect of zirconium oxide nanoparticles NPs on the levels of interleukins in mice inoculated with protoscoleces of Echinococcus granulosus, after exposure to ZrO2 NPs at the doses,5,10,50, and 100 µg /ml for 30 minutes, and different periods of infection, on the other hand, control groups inoculated with unexposed protoscoleces to the nanoparticles. Following the infection, blood serum was obtained from animals to determine interleukin levels using sandwich ELISA technique three, four, and five months following the infection. The outcome of the study demonstrated a significant decrease in IL-5, IL-12 levels, and a significant increase in IFN-γ- levels in treated mice compared with control groups. The lowest value of IL-5 was 11.164 ng /ml at the dose of 100 µg /ml compared to the control group 47.446 ng /ml, four months after infestation, while the lowest value for IL-12 was 12.824 ng/ml, at 100 µg /ml, compared to 112.485 ng/mL in the control group, five months to infection. IFN-γ was significantly increased in the treated mice, 207.39 ng/ml, at 100 µg /ml, compared to 110.07 ng/mL in the control group, five months after injury. Based on the research outcome, ZrO2 nanoparticles could be exploited as a consequential titer for assessing the immune situation and, consequently as a dynamic curative of hydatidosis.

Keywords

• hydatidosis
• interleukin-12
• interferon-γ