Mangalitsa Germoplasm Preservation Using Immature Oocyte Vitrification in SOPS

Abstract

The aim of this study was to determine the effectiveness of using two different concentrations of cryoprotectant following vitrification of immature oocytes. We used 1351 Mangalitsa oocytes for SOPS vitrification at an ethylene glycol (EG) concentration of 40% and 45% with an exposure time of 40 and 50 seconds. After warming, oocytes were subjected to morphological and viability assessment. The results show that increasing of EG concentration and exposure time of immature oocyte increases the number of detached cumulus cells and abnormal ooplasm. However, fluorescent assay indicated that a higher concentration of EG and shorter exposure time of oocytes lead to a significantly better post-vitrification viability (p > 0.001).

Keywords

• immature oocytes
• mangalitsa
• sops
• vitrification