Nitric oxide promotes differentiation of rat white preadipocytes in culture

Hongyun Yan; Edith Aziz; Gillian Shillabeer; Alex Wong; Deena Shanghavi; Abdenaim Kermouni; Mohammed Abdel-Hafez; David C.W. Lau

Journal of Lipid Research (Dec 2002)

Nitric oxide promotes differentiation of rat white preadipocytes in culture

Hongyun Yan,
Edith Aziz,
Gillian Shillabeer,
Alex Wong,
Deena Shanghavi,
Abdenaim Kermouni,
Mohammed Abdel-Hafez,
David C.W. Lau

Affiliations

Hongyun Yan: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Edith Aziz: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Gillian Shillabeer: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Alex Wong: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Deena Shanghavi: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Abdenaim Kermouni: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
Mohammed Abdel-Hafez: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada
David C.W. Lau: Julia McFarlane Diabetes Research Centre, Departments of Medicine, Biochemistry and Molecular Biology, The University of Calgary, Calgary, AB, T2N 4N1; Department of Medicine and the Loeb Health Research Institute, The Ottawa Hospital and the University of Ottawa, Ottawa, ON, K1Y 4E9, Canada

Journal volume & issue: Vol. 43, no. 12
pp. 2123 – 2129

Abstract

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The putative role of nitric oxide (3333333333333333335) in modulating adipogenesis was investigated in cultured preadipocytes derived from rat white adipose tissue. The NO releasing reagent, hydroxylamine (HA), and nitric oxide synthase (NOS) substrate l-arginine (Arg) had no influence on cell replication. However, both HA and Arg exhibited significant induction on differentiation, as evidenced by increased lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (GPDH) activities, as well as accelerated triacylglycerol (TG) accumulation. These observations suggested a positive role of NO in modulating adipogenesis. Preadipocytes were found to produce NO, and a ∼50% increase over basal level was observed on the first 2 days of differentiation. Deprivation of endogenous NOS activity by a non-selective NOS inhibitor, NG-monomethyl-l-arginine (NMMA), partially abrogated the differentiation process, implicating a role for endogenous NO to stimulate preadipocyte differentiation. Both NOS isoforms, eNOS and iNOS, were detected in differentiating preadipocytes.Specific iNOS inhibitors (1400W and aminoguanidine) had little influence on NO production and differentiation, suggesting that eNOS rather than iNOS may be the major isoform involved in modulating adipogenesis.

Published in Journal of Lipid Research

ISSN: 0022-2275 (Print); 1539-7262 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry
Website: https://www.journals.elsevier.com/journal-of-lipid-research

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