Selenium nanoparticles effects on stearoyl-CoA desaturase gene transcription rate in male lamb

Abstract

Selenium play a crucial role in the formation of selenoprotein, hormones and intrinsic factors. The stearoyl-CoA desaturase enzyme (SCD) is present in many tissues of the body and its activity leads to the formation of a single-stranded fatty acid from saturated fatty acids. Present study was carried out on fat tail weaning male lambs to evaluate the effect of oral selenium nanoparticles administration on transcription rate of stearoyl-CoA desaturase enzyme. For this purpose, 14 male lambs were selected at weaning time and divided into three groups: treatment 1 (5 heads), treatment 2 (5 heads) and control (4 heads). Selenium nanoparticles were administered for 20 consecutive days at a dose of 0.05 and 0.1 mg/kg B.W. to treatment groups 1 and 2, respectively and distilled water to the control ones. Tail fat tissue samples were collected at days 0, 20 and 30 via core biopsy needle gauge 14. Transcription rate of stearoyl-CoA desaturase (SCD) enzyme gene was performed by RT-PCR method and data were analyzed statistically using ANOVA test at the level of p

Keywords

• selenium nanoparticles
• stearoyl coa enzyme
• tail fat tissue
• lamb