Acta Biomedica Scientifica (Feb 2013)
Dophamine-dependent disorder of conduct of albino rats with sublimate intoxication in the test of extrapolation deliverance
Abstract
The article presents the results of the examination, of albino rats in the test of extrapolation deliverance (TED) after sublimate intoxication, and madopar-125 injection with previous sublimate intoxication. The aim. of the work was comparative study of conduct of albino rats with sublimate intoxication and at the influence of ma-dopar-125 in combination with sublimate intoxication, in TED. We used TED method in the research that allows to study cognitive abilities of an animal in stress situation. The results were processed with, software package Statistica 6.0 with use of non-parametric Mann — Whitney parameter. Sublimate was introduced subcutaneously in form, of physiological solution in dose 2 mg/kg during 3 days. In 3 days after ending of sublimate explosion we introduced, physiological solution to the rats of the first group 60 minutes before testing and. the rats of the second, group had intraperitoneal introduction, of madopar-125 that was suspended in 0,9 % NaCl with 125 mg/ kg tween-80 in dose 0,6 ml/100 g of animal's body weight. Animals of control group had physiological solution in the same regimen. It was revealed that in 4 days after the ending of sublimate introduction to albino rats experimental animals in comparison with intact ones had significant increase of number of aversive reactions. After sublimate explosion almost one third of albino rats didn't dive under cylinder's edge. In experimental rats average and total duration of immobilization increased while average duration of clambering on cylinder's walls decreased. Latent period of first movement increased sharply both after toxic influence and after introduction of madopar-125 to the rats with sublimate intoxication in comparison with control animals. Introduction of madopar-125 to the rats with sublimate intoxication completely disturbed conduct of deliverance: no animals dove under cylinder's edge. Albino rats with madopar-125 introduction had significant increase of clambering on cylinder's walls in comparison with animals exposed by sublimate without madopar-125 introduction. Besides the number, average and total duration of aversive reactions in form of jumps in comparison, with control group and with rats after sublimate explosion without pharmacological influence significantly increased.
