Genotoxic Evaluation of Fe<sub>3</sub>O<sub>4</sub> Nanoparticles in Different Three Barley (<i>Hordeum vulgare</i> L.) Genotypes to Explore the Stress-Resistant Molecules
Inese Kokina,
Ilona Plaksenkova,
Renata Galek,
Marija Jermaļonoka,
Elena Kirilova,
Vjaceslavs Gerbreders,
Marina Krasovska,
Eriks Sledevskis
Affiliations
Inese Kokina
Department of Biotechnology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Ilona Plaksenkova
Department of Biotechnology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Renata Galek
Department of Genetics, Plant Breeding and Seed Science, Wroclaw University of Environmental and Life Sciences, Grunwaldzki Sq. 24A, 50-363 Wroclaw, Poland
Marija Jermaļonoka
Department of Biotechnology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Elena Kirilova
Department of Applied Chemistry, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Vjaceslavs Gerbreders
Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Marina Krasovska
Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Eriks Sledevskis
Department of Technology, Institute of Life Sciences and Technology, Daugavpils University, Parades Str. 1A, LV-5401 Daugavpils, Latvia
Sustainable agricultural practices are still essential due to soil degradation and crop losses. Recently, the relationship between plants and nanoparticles (NPs) attracted scientists’ attention, especially for applications in agricultural production as nanonutrition. Therefore, the present research was carried out to investigate the effect of Fe3O4 NPs at low concentrations (0, 1, 10, and 20 mg/L) on three genotypes of barley (Hordeum vulgare L.) seedlings grown in hydroponic conditions. Significant increases in seedling growth, enhanced chlorophyll quality and quantity, and two miRNA expression levels were observed. Additionally, increased genotoxicity was observed in seedlings grown with NPs. Generally, Fe3O4 NPs at low concentrations could be successfully used as nanonutrition for increasing barley photosynthetic efficiency with consequently enhanced yield. These results are important for a better understanding of the potential impact of Fe3O4 NPs at low concentrations in agricultural crops and the potential of these NPs as nanonutrition for barley growth and yield enhancement. Future studies are needed to investigate the effect of these NPs on the expression of resistance-related genes and chlorophyll synthesis-related gene expression in treated barley seedlings.
