Role of Autophagy in the Radiosensitivity of Human Lung Adenocarcinoma A549 Cells

Chinese Journal of Lung Cancer. 2016;19(12):799-804 DOI 10.3779/j.issn.1009-3419.2016.12.01

 

Journal Homepage

Journal Title: Chinese Journal of Lung Cancer

ISSN: 1009-3419 (Print); 1999-6187 (Online)

Publisher: Chinese Anti-Cancer Association; Chinese Antituberculosis Association

Society/Institution: China Association for Science and Technology

LCC Subject Category: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens

Country of publisher: China

Language of fulltext: Chinese

Full-text formats available: PDF, HTML

 

AUTHORS


Liyao XU (Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

Yong WANG (Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

Qin LIU (Department of Respirology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

Hui LUO (Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

Xiaojun ZHONG (Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

Yong LI (Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China)

EDITORIAL INFORMATION

Peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 14 weeks

 

Abstract | Full Text

Background and objective Radiotherapy is an important treatment for lung cancer. The poor prognosis of lung cancer is largely caused by the high recurrence rate and metastasis of the tumor. Autophagy, which can be induced by radiotherapy, might be associated with DNA repair. The aim of this study is to investigate whether activating autophagy using rapamycin can enhance the radiosensitivity of lung cancer cells and clarify the association of autophagy with DNA repair. Methods The human adenocarcinoma A549 cell line was selected as the experimental subject. The specimens were divided into three groups: control (N), radiation (R), and Rapamycin and radiation (R+RAPA). The protein levels of γ-H2AX, Rad51, Ku70/Ku80, p62, and LC3 were determined by Western blot. Autophagosome was observed under a transmission electron microscope, and SF was determined by colony formation assay. Results Compared with group R, the activity of autophagy and the protein expression levels of Rad51 and Ku70/80 were remarkably increased in group R+RAPA. Conclusion The radiosensitivity of lung cancer can be promoted by activating autophagy via treatment with Rapamycin, and the process may be associated with DNA repair.