The smfBox is an open-source platform for single-molecule FRET

Benjamin Ambrose; James M. Baxter; John Cully; Matthew Willmott; Elliot M. Steele; Benji C. Bateman; Marisa L. Martin-Fernandez; Ashley Cadby; Jonathan Shewring; Marleen Aaldering; Timothy D. Craggs

doi:10.1038/s41467-020-19468-4

Nature Communications (Nov 2020)

The smfBox is an open-source platform for single-molecule FRET

Benjamin Ambrose,
James M. Baxter,
John Cully,
Matthew Willmott,
Elliot M. Steele,
Benji C. Bateman,
Marisa L. Martin-Fernandez,
Ashley Cadby,
Jonathan Shewring,
Marleen Aaldering,
Timothy D. Craggs

Affiliations

Benjamin Ambrose: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
James M. Baxter: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
John Cully: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
Matthew Willmott: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
Elliot M. Steele: Department of Physics, University of Sheffield
Benji C. Bateman: Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory
Marisa L. Martin-Fernandez: Central Laser Facility, Research Complex at Harwell, Rutherford Appleton Laboratory
Ashley Cadby: Department of Physics, University of Sheffield
Jonathan Shewring: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
Marleen Aaldering: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield
Timothy D. Craggs: Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield

DOI: https://doi.org/10.1038/s41467-020-19468-4
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 6

Abstract

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Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies between dyes on freely diffusing single molecules.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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