Methanolic extract of Buchholzia coriacea seed (MEBCS) attenuates lipopolysaccharide-induced perturbations of energy metabolizing enzymes in mice

Abstract

The safety of lipopolysaccharide (LPS)-containing foods has been questioned and the concerns are understandable. Buchholzia coriacea seeds have been widely applied in folkloric medicine due to their nutritional and therapeutic potential. The aim of the study is to evaluate the attenuation of methanolic extract of Buchholzia coriacea seed (MEBCS) on LPS-induced energy metabolism perturbations. Eighteen BALB/c mice were divided into three groups of 6 mice each. Group 1 (control) received distilled water and physiological saline for 7 days. Group 2 (LPS only) received 4 mg/Kg LPS intraperitoneally on the 7th day of administration, while Group 3 (LPS+MEBCS) were pre-treated with 250 mg/kg MEBCS orally for 6 days prior to administration of LPS (4 mg/kg) on the 7th day. The experimental animals were sacrificed afterward. Glycolytic and oxidative phosphorylation enzymes were assessed in the heart, kidney, spleen, lymphocytes, and erythrocytes. Glycolytic enzymes (lactate dehydrogenase, hexokinase, aldolase, and NADase) and oxidative phosphorylation enzymes such malate dehydrogenase, succinate dehydrogenase and electron transport complexes I+III, II+III, and IV) activities were significantly decreased (P> 0.05) by LPS. Pre-treatment with MEBCS attenuates the decrease in activity observed. The findings of this study indicated that MEBCS has the potential of attenuating perturbations of energy metabolism induced by lipopolysaccharides.

Keywords

• Buchholzia coriacea seed
• Mitochondria
• Glycolytic enzymes
• Oxidative phosphorylation
• Lipopolysaccharide