Sensors (May 2021)

Pursuing the Diffraction Limit with Nano-LED Scanning Transmission Optical Microscopy

  • Sergio Moreno,
  • Joan Canals,
  • Victor Moro,
  • Nil Franch,
  • Anna Vilà,
  • Albert Romano-Rodriguez,
  • Joan Daniel Prades,
  • Daria D. Bezshlyakh,
  • Andreas Waag,
  • Katarzyna Kluczyk-Korch,
  • Matthias Auf der Maur,
  • Aldo Di Carlo,
  • Sigurd Krieger,
  • Silvana Geleff,
  • Angel Diéguez

DOI
https://doi.org/10.3390/s21103305
Journal volume & issue
Vol. 21, no. 10
p. 3305

Abstract

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Recent research into miniaturized illumination sources has prompted the development of alternative microscopy techniques. Although they are still being explored, emerging nano-light-emitting-diode (nano-LED) technologies show promise in approaching the optical resolution limit in a more feasible manner. This work presents the exploration of their capabilities with two different prototypes. In the first version, a resolution of less than 1 µm was shown thanks to a prototype based on an optically downscaled LED using an LED scanning transmission optical microscopy (STOM) technique. This research demonstrates how this technique can be used to improve STOM images by oversampling the acquisition. The second STOM-based microscope was fabricated with a 200 nm GaN LED. This demonstrates the possibilities for the miniaturization of on-chip-based microscopes.

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