Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish
Tanveer Teranikar,
Cameron Villarreal,
Nabid Salehin,
Toluwani Ijaseun,
Jessica Lim,
Cynthia Dominguez,
Vivian Nguyen,
Hung Cao,
Cheng–Jen Chuong,
Juhyun Lee
Affiliations
Tanveer Teranikar
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Cameron Villarreal
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Nabid Salehin
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Toluwani Ijaseun
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Jessica Lim
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Cynthia Dominguez
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Vivian Nguyen
Martin High School/ UT Arlington, Arlington, TX, USA
Hung Cao
Department of Electrical Engineering, UC Irvine, Irvine, CA, USA
Cheng–Jen Chuong
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA
Juhyun Lee
Joint Department of Bioengineering, UT Arlington/UT Southwestern, Arlington, TX, USA; Department of Medical Education, TCU and UNTHSC School of Medicine, Fort Worth, TX 76107, USA; Corresponding author
Summary: In vivo quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders. In this regard, fixed tissue analysis has offered revolutionary insights into the underlying cellular architecture. However, histological analysis faces major drawbacks with respect to lack of spatiotemporal sampling and tissue artifacts during sample preparation. This study demonstrates the potential of light sheet fluorescence microscopy (LSFM) as a non-invasive, 4D (3days + time) optical sectioning tool for revealing cardiac mechano-transduction in zebrafish. Furthermore, we have described the utility of a scale and size-invariant feature detector, for analyzing individual morphology of fused cardiomyocyte nuclei and characterizing zebrafish ventricular contractility.
