X-ray crystallographic characterization of the SARS-CoV-2 main protease polyprotein cleavage sites essential for viral processing and maturation

Jaeyong Lee; Calem Kenward; Liam J. Worrall; Marija Vuckovic; Francesco Gentile; Anh-Tien Ton; Myles Ng; Artem Cherkasov; Natalie C. J. Strynadka; Mark Paetzel

doi:10.1038/s41467-022-32854-4

Nature Communications (Sep 2022)

X-ray crystallographic characterization of the SARS-CoV-2 main protease polyprotein cleavage sites essential for viral processing and maturation

Jaeyong Lee,
Calem Kenward,
Liam J. Worrall,
Marija Vuckovic,
Francesco Gentile,
Anh-Tien Ton,
Myles Ng,
Artem Cherkasov,
Natalie C. J. Strynadka,
Mark Paetzel

Affiliations

Jaeyong Lee: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Calem Kenward: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Liam J. Worrall: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Marija Vuckovic: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Francesco Gentile: Vancouver Prostate Centre, The University of British Columbia
Anh-Tien Ton: Vancouver Prostate Centre, The University of British Columbia
Myles Ng: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Artem Cherkasov: Vancouver Prostate Centre, The University of British Columbia
Natalie C. J. Strynadka: Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia
Mark Paetzel: Department of Molecular Biology and Biochemistry, Simon Fraser University

DOI: https://doi.org/10.1038/s41467-022-32854-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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The SARS-CoV-2 protease Mpro is essential for viral replication. Here, the authors have determined the structures of Mpro in complex with 10 of the 11 viral cleavage sequences including a covalent acyl-enzyme intermediate, providing mechanistic and structural insights for antiviral development.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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