Preventive effect of injections of vitamin D on the levels of Klotho in the brain tissue of female lewis rats with experimental autoimmune ensephalomyelitis

Abstract

Introdution: The aim of this study was to investigate the effect of vitamin D supplementation on Klotho levels of the brain tissue. Methods: In this laboratory experimental study, 25 female Lewis rats, after 2 weeks adaptation to lab environmental, were randomly divided into 5 groups: 1-control; 2- control+ EAE; 3-sham; 4vitamin D; 5-vitaminD + EAE. Results: The data were analyzed  using one way ANOVA, and to examine the weight changes Mann-Whitney test was used to the research groups. In order to induce EAE, 0.4 ml of solution, a combination of guinea Hindi pig spinal cord and adjuvant ratio was injected to each animal. Klotho levels were measured by ELISA. Finding: There was significantly different in Klotho levels of brain tissue of control+ EAE compared to control group (P=0/001). There was significant difference in Klotho levels of the brain tissue in EAE +vitamin D group compared to control group (P=0/001). In addition, the weight of vitamin D+EAE group was more than control+EAE group (P=0/006). Conclusion: Pre-treatment with vitamin D  supplementation increases the Klotho levels of brain tissue and may have protective role against EAE.

Keywords

• Experimental autoimmune encephalomyelitis
• Vitamin D
• Klotho.