A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes

Fernando López-Redondo; Junko Kurokawa; Fumimasa Nomura; Tomoyuki Kaneko; Tomoyo Hamada; Tetsushi Furukawa; Kenji Yasuda

doi:10.1016/j.jphs.2016.04.015

Journal of Pharmacological Sciences (Jun 2016)

A distribution analysis of action potential parameters obtained from patch-clamped human stem cell-derived cardiomyocytes

Fernando López-Redondo,
Junko Kurokawa,
Fumimasa Nomura,
Tomoyuki Kaneko,
Tomoyo Hamada,
Tetsushi Furukawa,
Kenji Yasuda

Affiliations

Fernando López-Redondo: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan
Junko Kurokawa: Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan
Fumimasa Nomura: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan
Tomoyuki Kaneko: Department of Frontier Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan
Tomoyo Hamada: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan
Tetsushi Furukawa: Department of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, 113-8510, Japan
Kenji Yasuda: Department of Biomedical Information, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, 101-0062, Japan

DOI: https://doi.org/10.1016/j.jphs.2016.04.015
Journal volume & issue: Vol. 131, no. 2
pp. 141 – 145

Abstract

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We investigated electrophysiological properties of human induced-pluripotent-stem-cell-derived and embryonic-stem-cell-derived cardiomyocytes, and analyzed action potential parameters by plotting their frequency distributions. In the both cell lines, the distribution analysis revealed that histograms of maximum upstroke velocity showed two subpopulations with similar intersection values. Sub-populations with faster maximum upstroke velocity showed significant prolongation of action potential durations by application of E-4031, whereas others did not, which may be partly due to shallower maximum diastolic potentials. We described electrophysiological and pharmacological properties of stem-cell-derived cardiomyocytes in the respective sub-populations, which provides a way to characterize diverse electrical properties of stem-cell-derived cardiomyocytes systematically.

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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