Nature Communications (Jan 2020)
Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics
- Julia Ast,
- Anastasia Arvaniti,
- Nicholas H. F. Fine,
- Daniela Nasteska,
- Fiona B. Ashford,
- Zania Stamataki,
- Zsombor Koszegi,
- Andrea Bacon,
- Ben J. Jones,
- Maria A. Lucey,
- Shugo Sasaki,
- Daniel I. Brierley,
- Benoit Hastoy,
- Alejandra Tomas,
- Giuseppe D’Agostino,
- Frank Reimann,
- Francis C. Lynn,
- Christopher A. Reissaus,
- Amelia K. Linnemann,
- Elisa D’Este,
- Davide Calebiro,
- Stefan Trapp,
- Kai Johnsson,
- Tom Podewin,
- Johannes Broichhagen,
- David J. Hodson
Affiliations
- Julia Ast
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Anastasia Arvaniti
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Nicholas H. F. Fine
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Daniela Nasteska
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Fiona B. Ashford
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Zania Stamataki
- Centre for Liver Research, College of Medical and Dental Sciences, Institute for Immunology and Immunotherapy, University of Birmingham
- Zsombor Koszegi
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Andrea Bacon
- Genome Editing Facility, Technology Hub, University of Birmingham
- Ben J. Jones
- Division of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London
- Maria A. Lucey
- Division of Diabetes, Endocrinology and Metabolism, Section of Investigative Medicine, Imperial College London
- Shugo Sasaki
- Diabetes Research Group, BC Children’s Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia
- Daniel I. Brierley
- Centre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London
- Benoit Hastoy
- Oxford Centre for Diabetes, Endocrinology & Metabolism, University of Oxford
- Alejandra Tomas
- Division of Diabetes, Endocrinology and Metabolism, Section of Cell Biology and Functional Genomics, Imperial College London
- Giuseppe D’Agostino
- Faculty of Biology, Medicine and Health, University of Manchester
- Frank Reimann
- Wellcome Trust-MRC Institute of Metabolic Science, University of Cambridge
- Francis C. Lynn
- Diabetes Research Group, BC Children’s Hospital Research Institute, Vancouver, BC, Canada; Department of Surgery, University of British Columbia
- Christopher A. Reissaus
- Department of Pediatrics, Indiana University School of Medicine
- Amelia K. Linnemann
- Department of Pediatrics, Indiana University School of Medicine
- Elisa D’Este
- Optical Microscopy Facility, Max Planck Institute for Medical Research
- Davide Calebiro
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- Stefan Trapp
- Centre for Cardiovascular and Metabolic Neuroscience, Department of Neuroscience, Physiology & Pharmacology, University College London
- Kai Johnsson
- Department of Chemical Biology, Max Planck Institute for Medical Research
- Tom Podewin
- Department of Chemical Biology, Max Planck Institute for Medical Research
- Johannes Broichhagen
- Department of Chemical Biology, Max Planck Institute for Medical Research
- David J. Hodson
- Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham
- DOI
- https://doi.org/10.1038/s41467-020-14309-w
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 18
Abstract
Glucagon-like peptide-1 receptor is an important regulator of appetite and glucose homeostasis. Here the authors describe super-resolution microscopy and in vivo imaging compatible fluorescent probes, which reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics in islets and brain.
