Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States
Gaoxiang Liu
Advanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
Cheng Lyu
Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States
Sayeh Kohani
Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States
Qijing Xie
Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States
David J Luginbuhl
Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, United States
Srigokul Upadhyayula
Advanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, United States; Chan Zuckerberg Biohub, San Francisco, United States
Eric Betzig
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, United States; Advanced Bioimaging Center, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States; Departments of Molecular and Cell Biology and Physics, Howard Hughes Medical Institute, Helen Wills Neuroscience Institute, University of California, Berkeley, United States
How does wiring specificity of neural maps emerge during development? Formation of the adult Drosophila olfactory glomerular map begins with the patterning of projection neuron (PN) dendrites at the early pupal stage. To better understand the origin of wiring specificity of this map, we created genetic tools to systematically characterize dendrite patterning across development at PN type–specific resolution. We find that PNs use lineage and birth order combinatorially to build the initial dendritic map. Specifically, birth order directs dendrite targeting in rotating and binary manners for PNs of the anterodorsal and lateral lineages, respectively. Two-photon– and adaptive optical lattice light-sheet microscope–based time-lapse imaging reveals that PN dendrites initiate active targeting with direction-dependent branch stabilization on the timescale of seconds. Moreover, PNs that are used in both the larval and adult olfactory circuits prune their larval-specific dendrites and re-extend new dendrites simultaneously to facilitate timely olfactory map organization. Our work highlights the power and necessity of type-specific neuronal access and time-lapse imaging in identifying wiring mechanisms that underlie complex patterns of functional neural maps.
