Nature Communications (Dec 2021)

Directed-evolution of translation system for efficient unnatural amino acids incorporation and generalizable synthetic auxotroph construction

  • Hongxia Zhao,
  • Wenlong Ding,
  • Jia Zang,
  • Yang Yang,
  • Chao Liu,
  • Linzhen Hu,
  • Yulin Chen,
  • Guanglong Liu,
  • Yu Fang,
  • Ying Yuan,
  • Shixian Lin

DOI
https://doi.org/10.1038/s41467-021-27399-x
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 12

Abstract

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In this paper, the authors report the directed-evolution of translation systems that allow the incorporation of unnatural amino acids with similar efficiency to natural amino acids and the construction of synthetic auxotroph in a generalizable way.