Impact of malathion toxicity on the oxidative stress parameters of the black soldier fly Hermetia illucens (Linnaeus, 1758) (Diptera: Stratiomyidae)

Abstract

Abstract The black soldier fly larvae (BSFL) may serve as a promising tool in the animals feed production industry. The input organic wastes may be contaminated by insecticides that affect both the insect’s mass rearing, and the animals feed process. Therefore, in the current study the assessment of oxidative stress parameters of the black soldier fly (BSF) were investigated to quantify the deleterious effect of malathion-contaminated kitchen waste (1:1 vegetable: fruit waste) container on the insect. The different developmental stages of insect (adult and larva) were exposed to different concentrations (0, 0.005, 0.01, 0.015, and 0.02 mg/mL) of malathion. The results showed that the mean value of the reactive oxygen species (ROS), which included hydrogen peroxide (H2O2) and superoxide anion radicals (O2•-) concentrations were lower in larval stage than in adults, in all treated groups (0, 0.005, 0.01, 0.015, and 0.02 mg/mL malathion concentration). Also, the protein carbonyls amount and lipid peroxides levels were decreased in the 0.02 mg/mL Malathion compared to the control values. However, the cluster analysis revealed slight dissimilar patterns for control insects and the highest malathion concentration (0.02 mg/ml). These stage-related differences could occur from the different growth dynamic functions of larvae and adults. The larvae were distinguished by robust growth, and significant oxygen consumption. The results verified that oxidative stress parameters, especially protein carbonyls and α, α-diphenyl-β-picrylhydrazyl (DPPH) were promising, cheap, quick and cost-effective applications for determining the macromolecules damage, and antioxidant ability of H. illucens enclosed with malathion exposure. These findings described that malathion application induces macromolecules damage mediated through oxidative stress injury.