Nature Communications (Oct 2022)
Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE
Abstract
Applications of the SCRaMbLE process are hindered due to the lack of facile and tight regulation and limited understanding of key factors that may affect the rearrangement outcomes. Here the authors present an approach to precisely regulate SCRaMbLE recombination in a dose-dependent manner using genetic code expansion technology with low basal activity.