Independent effect of main components in particulate matter on DNA methylation and DNA methyltransferase: A molecular epidemiology study

Yanhua Wang; Ting Wang; Mengmeng Xu; Haitao Yu; Chunguang Ding; Zhenjie Wang; Xingfu Pan; Yanbo Li; Yong Niu; Ruixue Yan; Jiayang Song; Huifang Yan; Yufei Dai; Zhiwei Sun; Wenge Su; Huawei Duan

Environment International (Jan 2020)

Independent effect of main components in particulate matter on DNA methylation and DNA methyltransferase: A molecular epidemiology study

Yanhua Wang,
Ting Wang,
Mengmeng Xu,
Haitao Yu,
Chunguang Ding,
Zhenjie Wang,
Xingfu Pan,
Yanbo Li,
Yong Niu,
Ruixue Yan,
Jiayang Song,
Huifang Yan,
Yufei Dai,
Zhiwei Sun,
Wenge Su,
Huawei Duan

Affiliations

Yanhua Wang: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Ting Wang: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Mengmeng Xu: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China; School of Public Health, Shandong University, Jinan, China
Haitao Yu: Laigang Hospital Affiliated to Taishan Medical University, Laiwu, China
Chunguang Ding: National Center for Occupational Safety and Health, National Health Commission of the People's Republic of China, Beijing, China
Zhenjie Wang: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Xingfu Pan: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Yanbo Li: School of Public Health, Capital Medical University, Beijing, China
Yong Niu: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Ruixue Yan: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Jiayang Song: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Huifang Yan: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Yufei Dai: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China
Zhiwei Sun: School of Public Health, Capital Medical University, Beijing, China
Wenge Su: Laigang Hospital Affiliated to Taishan Medical University, Laiwu, China
Huawei Duan: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China; Corresponding author at: Key Laboratory of Chemical Safety and Health, National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing 100050, China.

Journal volume & issue: Vol. 134

Abstract

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Background: There is a paucity of mechanistic information on the DNA methylation and particulate matter (PM) exposure. This study aimed to investigate the association of PM and its component with DNA methylation, and the roles of DNA methyltransferase (DNMTs). Methods: There were 240 high-exposed, 318 low-exposed and 210 non-exposed participants in this study. Individual concentrations of PM, polycyclic aromatic hydrocarbons (PAHs) and metals were identified by the monitoring data in their workplaces. Urinary 1-OHP and metals were determined as exposure markers. The global DNA methylation (% 5mC) and the mRNA expression of DNMT1, DNMT3A and DNMT3B were measured. We used mediation analysis to evaluate the role of DNMTs expression on DNA methylation alteration induced by PAHs and metals components. Results: The decreasing trend of % 5mC was associated with increment of PM exposure in all subjects. We found that one IQR increase in total PAHs (3.82 μg/m3) and urinary 1-OHP (1.06 μmol/mol creatinine) were associated with a separate 6.08% and 7.26% decrease in % 5mC (P = 0.009, P < 0.001), and one IQR increase in urinary Ni (27.75 μmol/mol creatinine) was associated with a 3.29% decrease in % 5mC (P = 0.03). The interaction of urinary 1-OHP with Ni on global DNA methylation (%5mC) was not found (P interaction = 0.89). PM exposure was significantly associated with decreased mRNA level of DNMT3B, but the mediated effect of the PAHs and Ni levels on % 5mC through the DNMT3B pathway was not observed. Conclusions: We found the decrement of global DNA methylation and DNMT3B expression with elevated PM levels in population. The independent mode of action on DNA hypomethylation was found from PAHs and metal components. Global DNA hypomethylation might be a potential biomarker for evaluation of adverse health effects in response to PM exposure. Keywords: Polycyclic aromatic hydrocarbons, Heavy metals, Global DNA methylation, DNA methyltransferase

Published in Environment International

ISSN: 0160-4120 (Print)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Geography. Anthropology. Recreation: Environmental sciences
Website: https://www.journals.elsevier.com/environment-international

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