Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch; Katharina Günther; Jonas Waider; Georg C. Ziegler; Andrea Forero; Sina Kollert; Evgeniy Svirin; Dirk Pühringer; Chee Keong Kwok; Reinhard Ullmann; Anna Maierhofer; Julia Flunkert; Thomas Haaf; Frank Edenhofer; Klaus-Peter Lesch

Stem Cell Research (Apr 2018)

Generation of a human induced pluripotent stem cell (iPSC) line from a 51-year-old female with attention-deficit/hyperactivity disorder (ADHD) carrying a duplication of SLC2A3

Charline Jansch,
Katharina Günther,
Jonas Waider,
Georg C. Ziegler,
Andrea Forero,
Sina Kollert,
Evgeniy Svirin,
Dirk Pühringer,
Chee Keong Kwok,
Reinhard Ullmann,
Anna Maierhofer,
Julia Flunkert,
Thomas Haaf,
Frank Edenhofer,
Klaus-Peter Lesch

Affiliations

Charline Jansch: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
Katharina Günther: Stem Cell and Regenerative Medicine Group, Institute of Anatomy and Cell Biology II, University of Würzburg, Würzburg, Germany
Jonas Waider: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
Georg C. Ziegler: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
Andrea Forero: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany
Sina Kollert: Institute of Physiology, Molecular Electrophysiology, University of of Würzburg, Würzburg, Germany
Evgeniy Svirin: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany; Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia
Dirk Pühringer: Stem Cell and Regenerative Medicine Group, Institute of Anatomy and Cell Biology II, University of Würzburg, Würzburg, Germany
Chee Keong Kwok: Stem Cell and Regenerative Medicine Group, Institute of Anatomy and Cell Biology II, University of Würzburg, Würzburg, Germany
Reinhard Ullmann: Bundeswehr Institute of Radiobiology Affiliated to the University of Ulm, Munich, Germany
Anna Maierhofer: Institute of Human Genetics, University of Würzburg, Würzburg, Germany
Julia Flunkert: Institute of Human Genetics, University of Würzburg, Würzburg, Germany
Thomas Haaf: Institute of Human Genetics, University of Würzburg, Würzburg, Germany
Frank Edenhofer: Stem Cell and Regenerative Medicine Group, Institute of Anatomy and Cell Biology II, University of Würzburg, Würzburg, Germany; Department of Genomics, Stem Cell Biology and Regenerative Medicine, Institute of Molecular Biology, CMBI, Leopold-Franzens-University Innsbruck, Innsbruck, Austria; Correspondence to: F. Edenhofer, Department of Genomics, Stem Cell Biology and Regenerative Medicine, Institute of Molecular Biology, CMBI, Leopold-Franzens-University Innsbruck, Technikerstraße 25, 6020 Innsbruck, Austria.
Klaus-Peter Lesch: Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Würzburg, Germany; Laboratory of Psychiatric Neurobiology, Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Moscow, Russia; Department of Translational Neuroscience, School for Mental Health and Neuroscience (MHeNS), Maastricht University, Maastricht, The Netherlands; Correspondence to: K. P. Lesch, Division of Molecular Psychiatry, Center of Mental Health, University of Würzburg, Margarete-Höppel-Platz 1, 97080 Würzburg, Germany.

Journal volume & issue: Vol. 28
pp. 136 – 140

Abstract

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Fibroblasts were isolated from a skin biopsy of a clinically diagnosed 51-year-old female attention-deficit/hyperactivity disorder (ADHD) patient carrying a duplication of SLC2A3, a gene encoding neuronal glucose transporter-3 (GLUT3). Patient fibroblasts were infected with Sendai virus, a single-stranded RNA virus, to generate transgene-free human induced pluripotent stem cells (iPSCs). SLC2A3-D2-iPSCs showed expression of pluripotency-associated markers, were able to differentiate into cells of the three germ layers in vitro and had a normal female karyotype. This in vitro cellular model can be used to study the role of risk genes in the pathogenesis of ADHD, in a patient-specific manner.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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