Frontiers in Immunology (Nov 2023)

High-dimensional phenotyping of the peripheral immune response in community-acquired pneumonia

  • Tom D. Y. Reijnders,
  • Alex R. Schuurman,
  • Jan Verhoeff,
  • Marlous van den Braber,
  • Renée A. Douma,
  • Daniël R. Faber,
  • Alberta G. A. Paul,
  • W. Joost Wiersinga,
  • W. Joost Wiersinga,
  • Anno Saris,
  • Juan J. Garcia Vallejo,
  • Tom van der Poll,
  • Tom van der Poll

DOI
https://doi.org/10.3389/fimmu.2023.1260283
Journal volume & issue
Vol. 14

Abstract

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BackgroundCommunity-acquired pneumonia (CAP) represents a major health burden worldwide. Dysregulation of the immune response plays an important role in adverse outcomes in patients with CAP.MethodsWe analyzed peripheral blood mononuclear cells by 36-color spectral flow cytometry in adult patients hospitalized for CAP (n=40), matched control subjects (n=31), and patients hospitalized for COVID-19 (n=35).ResultsWe identified 86 immune cell metaclusters, 19 of which (22.1%) were differentially abundant in patients with CAP versus matched controls. The most notable differences involved classical monocyte metaclusters, which were more abundant in CAP and displayed phenotypic alterations reminiscent of immunosuppression, increased susceptibility to apoptosis, and enhanced expression of chemokine receptors. Expression profiles on classical monocytes, driven by CCR7 and CXCR5, divided patients with CAP into two clusters with a distinct inflammatory response and disease course. The peripheral immune response in patients with CAP was highly similar to that in patients with COVID-19, but increased CCR7 expression on classical monocytes was only present in CAP.ConclusionCAP is associated with profound cellular changes in blood that mainly relate to classical monocytes and largely overlap with the immune response detected in COVID-19.

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