Cell Reports (Mar 2014)
A Chemical Probe that Labels Human Pluripotent Stem Cells
- Nao Hirata,
- Masato Nakagawa,
- Yuto Fujibayashi,
- Kaori Yamauchi,
- Asako Murata,
- Itsunari Minami,
- Maiko Tomioka,
- Takayuki Kondo,
- Ting-Fang Kuo,
- Hiroshi Endo,
- Haruhisa Inoue,
- Shin-ichi Sato,
- Shin Ando,
- Yoshinori Kawazoe,
- Kazuhiro Aiba,
- Koh Nagata,
- Eihachiro Kawase,
- Young-Tae Chang,
- Hirofumi Suemori,
- Koji Eto,
- Hiromitsu Nakauchi,
- Shinya Yamanaka,
- Norio Nakatsuji,
- Kazumitsu Ueda,
- Motonari Uesugi
Affiliations
- Nao Hirata
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Masato Nakagawa
- Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
- Yuto Fujibayashi
- Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
- Kaori Yamauchi
- Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
- Asako Murata
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Itsunari Minami
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Maiko Tomioka
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Takayuki Kondo
- Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
- Ting-Fang Kuo
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Hiroshi Endo
- Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
- Haruhisa Inoue
- Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
- Shin-ichi Sato
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Shin Ando
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Yoshinori Kawazoe
- Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan
- Kazuhiro Aiba
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Koh Nagata
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Eihachiro Kawase
- Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
- Young-Tae Chang
- Department of Chemistry & MedChem Program of Life Sciences Institute, National University of Singapore, Singapore 117543, Singapore
- Hirofumi Suemori
- Stem Cell Research Center, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan
- Koji Eto
- Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan
- Hiromitsu Nakauchi
- Laboratory of Stem Cell Therapy, Center for Experimental Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
- Shinya Yamanaka
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Norio Nakatsuji
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Kazumitsu Ueda
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- Motonari Uesugi
- Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University, Kyoto 606-8501, Japan
- DOI
- https://doi.org/10.1016/j.celrep.2014.02.006
- Journal volume & issue
-
Vol. 6,
no. 6
pp. 1165 – 1174
Abstract
A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.
